2001 TSSAA Division I Class AA State Softball Tournament
| Seeding | May 14 Regional Semi-Finals |
May 16 Regional Finals |
May 18 Sectional |
May 22-26 State | |||
| (H) Dist. 1 W | Unicoi Co. 12 | ||||||
| ¦ | Unicoi Co. 6 | ||||||
| Dist. 2 R-Up | Claiborne Co. 0 | ¦ | ¦ | ||||
| ¦ | Unicoi Co. 1 | ||||||
| (H) Dist. 2 W | Union Co. 3 | ¦ | ¦ | ||||
| ¦ | Union Co. 0 | ¦ | ¦ | Kingston | |||
| Dist. 1 R-Up | Volunteer 2 | ¦ | ¦ | ||||
| Kingston 3 | ¦ | ||||||
| (H) Dist. 3 W | Gibbs 3 | ||||||
| ¦ | Gibbs 4 | ||||||
| Dist. 4 R-Up | Anderson Co. 1 | ¦ | ¦ | ||||
| ¦ | Gibbs 5 | ||||||
| (H) Dist. 4 W | Kingston 2 | ¦ | ¦ | ||||
| ¦ | Kingston 2 | ¦ | ¦ | Gibbs | |||
| Dist. 3 R-Up | Carter 1 | ¦ | ¦ | ||||
| Union Co. 0 | ¦ | ||||||
| (H) Dist. 5 W | Sweetwater 8 | ||||||
| ¦ | Sweetwater 3 | ||||||
| Dist. 6 R-Up | Brainerd 2 | ¦ | ¦ | ||||
| ¦ | Chatt. Central 2 | ||||||
| (H) Dist. 6 W | Chatt. Central 16 | ¦ | ¦ | ||||
| ¦ | Chatt. Central 9 | ¦ | ¦ | Smith Co. | |||
| Dist. 5 R-Up | Sequoyah 1 | ¦ | ¦ | ||||
| Smith Co. 8 | ¦ | ||||||
| (H) Dist. 7 W | Sequatchie Co. 0 | ||||||
| ¦ | DeKalb Co. 9 | ||||||
| Dist. 8 R-Up | DeKalb Co. 1 | ¦ | ¦ | ||||
| ¦ | DeKalb Co. 2 | ||||||
| (H) Dist. 8 W | Smith Co. 7 | ¦ | ¦ | ||||
| ¦ | Smith Co. 4 | ¦ | ¦ | DeKalb Co. | |||
| Dist. 7 R-Up | Marion Co. 2 | ¦ | ¦ | ||||
| Sweetwater 0 | ¦ | ||||||
| (H) Dist. 9 W | David Lipscomb 6 | ||||||
| ¦ | David Lipscomb 6 | ||||||
| Dist. 10 R-Up | Macon Co. 0 | ¦ | ¦ | ||||
| ¦ | Lipscomb 2 | ||||||
| (H) Dist. 10 W | Greenbrier 3 | ¦ | ¦ | ||||
| ¦ | Greenbrier 0 | ¦ | ¦ | Lipscomb | |||
| Dist. 9 R-Up | Hume Fogg 0 | ¦ | ¦ | ||||
| Marshall Co. 0 | ¦ | ||||||
| (H) Dist. 11 W | Giles Co. 14 | ||||||
| ¦ | Giles Co. 5 | ||||||
| Dist. 12 R-Up | Montgomery Cent. 0 | ¦ | ¦ | ||||
| ¦ | Giles Co. 4 | ||||||
| (H) Dist. 12 W | Waverly 1 | ¦ | ¦ | ||||
| ¦ | Marshall Co. 0 | ¦ | ¦ | Giles Co. | |||
| Dist. 11 R-Up | Marshall Co. 2 | ¦ | ¦ | ||||
| Greenbrier 2 | ¦ | ||||||
| (H) Dist. 13 W | Covington 5 | ||||||
| ¦ | Covington 0 | ||||||
| Dist. 14 R-Up | Chester Co. 1 | ¦ | ¦ | ||||
| ¦ | Lexington 15 | ||||||
| (H) Dist. 14 W | Lexington 2 | ¦ | ¦ | ||||
| ¦ | Lexington 1 | ¦ | ¦ | Lexington | |||
| Dist. 13 R-Up | Brighton 1 | ¦ | ¦ | ||||
| Carver 0 | ¦ | ||||||
| (H) Dist. 15 W | Ridgeway 18 | ||||||
| ¦ | Ridgeway 16 | ||||||
| Dist. 16 R-Up | B.T. Washington 4 | ¦ | ¦ | ||||
| ¦ | Ridgeway 0 | ||||||
| (H) Dist. 16 W | Carver 10 | ¦ | ¦ | ||||
| ¦ | Carver 10 | ¦ | ¦ | Covington | |||
| Dist. 15 R-Up | Frayser 9 | ¦ | ¦ | ||||
| Covington 18 | ¦ |
If a district winner from a district that is to host the regional finals is defeated, the district runner-up from that district shall host the regional finals. (Example: If it is district 2's time to host the region and the district 2 winner is defeated, the district 2 runner-up (provided that team wins) shall host the regional finals. If both teams from district are defeated in the regional semi-final games, the district 1 winner shall host the regional finals.)